Louis A. Ciampi was born in Portland, Maine into a family where he was the youngest of eleven children. His parents, Michael and Marianna, were immigrants from Italy who spoke little English, but wanted to give their children a chance to achieve what would not have been possible in the Old World. With the support of his family and a lot of hard work of his own, he worked his way through college at St. Francis Xavier in Antigonish, Nova Scotia. He also worked to pay his way through medical school as well. He earned his Doctor of Medicine degree from Dalhousie University in Halifax, Nova Scotia in 1958.
After his internship, he returned to his native Maine to establish himself. He practiced for a few years in the town of Gray before relocating to his hometown of Portland.